AJ-FLUOR: Fluorescent particles

A fluorescent dye is incorporated in the particles in order to magnify the agglutination signal. The fluorophore (the group responsible for fluorescence) is excited to a higher energy state by an external light at a certain wavelength (λex) and after some conformational changes in the fluorescent molecule, light is emitted at a higher wavelength (λem). In practice, the presence of agglutinated fluorescent particles in diagnostic tests can be detected exciting them and measuring the intensity of the light emitted with an appropriate detector. The sensitivity of this type of detection is higher than other techniques based on absorbance, colorimetry…

AJ-Fluor

Variables

  • Particle size: from 50 to 2000 nm.
  • Fluorescence properties (λex and λem):
    λex (nm) λem (nm)
    1 375 427
    2 477 519
    3 481 524
    4 492 536
    5 548 568
    6 576 596
    * Other fluorophores available upon request
  • Fluorescent intensity: low and high.
  • Functionality: -COOH, -CH2Cl, -SO3-, -NH+
  • Parking area of the functional group: from 15 to 200 Å2/group.
  • Cleaning process.

Uses

AJ-Fluor particles are used in immunochromatography or slide agglutination diagnostic kits in which high sensitivity is desired. They can be used as markers too.

Examples of reference codes

  • AJ20COOH-F427: Mean particle size 200 nm. Surface carboxyl groups. Fluorescent latex with λem=427nm.